Methods in Cell Biology
نویسندگان
چکیده
I. Why Tubulin from Nonneural Sources?II. General Principles of Tubulin PurificationIII. Specific Properties of Distinct Nonneural SourcesA. Metazoan Sources (e.g., Tissue Culture Cells, Chicken Red Blood Cells)B. Tetrahymena thermophilaC. Kinetoplastid Parasites (Leishmania, Trypanosoma)D. Saccharomyces cerevisiaeE. Land PlantsIV. Genetic Manipulation of Tubulin GenesA. Genetic Manipulation of Tubulin Genes in S. cerevisiaeB. Genetic Manipulation of Tubulin Genes in T. thermophilaV. Isolation of Tubulin: Theme and VariationVI. Protocol NotesVII. SummaryAcknowledgmentsReferences
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